TDG Protein (His tag)

Produktnummer ABIN1098673

Produktdetails

Target: TDG (Thymine-DNA Glycosylase (TDG))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses TDG Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 Urea, 10% glycerol, 0.1 M NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: TDG (Thymine-DNA Glycosylase (TDG))

Andere Bezeichnung: TDG (TDG Produkte)

Hintergrund: Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E.coli

Molekulargewicht: 48.4 kDa (433aa)

Pathways: DNA Reparatur, Chromatin Binding