LIF Protein (AA 23-202)

Produktnummer ABIN1344370

Produktdetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 23-202

Spezies: Human, Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Spezifität: Mouse and human embryonic stem cells.

Kreuzreaktivität: Human, Maus

Produktmerkmale: Human LIF (aa 23-202) is fused at the N-terminus to a tag.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.05EU/μg purified protein (LAL test, Lonza).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Andere Bezeichnung: LIF (LIF Produkte)

Synonyme: CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein

Hintergrund: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.

Molekulargewicht: ~46kDa (SDS-PAGE)

UniProt: P15018

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding