Flagellin Protein (FliC)

Produktnummer ABIN1344457

Produktdetails

Target: Flagellin (FliC)

Protein-Typ: Native

Spezies: Salmonella typhimurium

Quelle: Salmonella typhimurium

Verwendungszweck: Flagellin (high purity)

Spezifität: Binds to human and mouse TLR5 (Toll-like receptor 5).

Kreuzreaktivität: Human, Maus

Reinheit: high purity

Endotoxin-Niveau: <0.001EU/μg purified protein (LAL test, Lonza).

Biological Activity Comment: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.1 mg/mL after reconstitution

Konzentration: 0.1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: Flagellin (FliC)

Andere Bezeichnung: Flagellin (FliC Produkte)

Hintergrund:

FliC

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

Molekulargewicht: ~54kDa (SDS-PAGE)

UniProt: P06179

Pathways: Inflammasome