CDNF Protein

Produktnummer ABIN1405917

Produktdetails

Target: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: CHO Cells

Applikation: Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)

Produktmerkmale: Biological properties: Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007, Voutilainen et al. 2011)

Aufreinigung: Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Protein is sterile-filtrated through 0.22 µg filter.
Purified human CDNF is characterized as homogenous material by mass-spectroscopy.

Endotoxin-Niveau: Less than 1EU/µg of protein as determined by LAL method

Anwendungsinformationen

Kommentare:

Synonyms: Cerebral dopamine neurotrophic factor - CDNF, ARMETL1.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer: PBS pH 7.5

Handhabung: Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Antigendetails

Target: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Andere Bezeichnung: CDNF (CDNF Produkte)

Hintergrund: CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007, Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Molekulargewicht: Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

UniProt: Q49AH0