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- Target Alle Protein A Proteine anzeigen
- Protein A
- Spezies
- Staphylococcus aureus
- Wirt
- Bitte anfragen
- Aufreinigungstag / Konjugat
- Dieses Protein A Protein ist gelabelt mit Biotin.
- Applikation
- ELISA, Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC)
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- Applikationshinweise
- Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:5,000-1:50,000 Western blot: 1:5,000-1:50,000 ELISA: 1:50,000-1:500,000 Other: user-optimized.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 2 mg/ml
- Buffer
- PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.
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Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).
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Specific antibody immobilization with biotin-poly(L-lysine)-g-poly(ethylene glycol) and protein A on microfluidic chips." in: Journal of immunological methods, Vol. 350, Issue 1-2, pp. 97-105, (2009) (PubMed).
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- Target
- Protein A
- Abstract
- Protein A Produkte
- Synonyme
- alpha protein Protein, repB Protein
- Hintergrund
- Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
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