IL-1 beta Protein
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- Target Alle IL-1 beta (IL1B) Proteine anzeigen
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- IL-1 beta
- Sequenz
- APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
- Spezifität
- Chromosomal location:2q14
- Produktmerkmale
- Length (aa):153
- Reinheit
- > 98 % by SDS-PAGE
- Endotoxin-Niveau
- < 0.1 ng per μg (IEU/μg) of rh IL-1ß
- Top Product
- Discover our top product IL1B Protein
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- Applikationshinweise
- Measured in a cell proliferation assay using murine D10G4.1 cells. The ED50 for this effect is typically 2-10 pg/mL.
- Kommentare
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Cytokines & Growth Factors
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- The lyophilized rh IL-1ß is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
- Buffer
- PBS
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Lyophilized IL-1ß although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-1ß should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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Regulation of the hyperosmotic induction of aquaporin 5 and VEGF in retinal pigment epithelial cells: involvement of NFAT5." in: Molecular vision, Vol. 21, pp. 360-77, (2015) (PubMed).
: "Mutant p53 gains new function in promoting inflammatory signals by repression of the secreted interleukin-1 receptor antagonist." in: Oncogene, Vol. 34, Issue 19, pp. 2493-504, (2015) (PubMed).
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Regulation of the hyperosmotic induction of aquaporin 5 and VEGF in retinal pigment epithelial cells: involvement of NFAT5." in: Molecular vision, Vol. 21, pp. 360-77, (2015) (PubMed).
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- Target
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- Andere Bezeichnung
- IL-1 beta (IL1B Produkte)
- Synonyme
- IL-1 Protein, IL1-BETA Protein, IL1F2 Protein, IL-1BETA Protein, IL1beta Protein, il1-b Protein, zgc:111873 Protein, IL-1B Protein, IL-1beta Protein, Il-1b Protein, IL1B Protein, IL-1 beta Protein, IL-1b Protein, interleukin 1 beta Protein, interleukin 1, beta Protein, IL1B Protein, il1b Protein, Il1b Protein
- Hintergrund
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Interleukin-1 beta (IL-1beta) is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL1F1) and IL1 beta (IL1F2), which are the products of distinct genes. IL- 1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21 % amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta converting enzyme (Caspase1/ICE) to generate the active cytokine. The mature human IL-1 beta shares 96 % aa sequence identity with rhesus and 67% 78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. Human recombinant IL-1beta produced in E. coli is a non-glycosylated, IL-1 beta polypeptide chain containing 153 amino acids and having a molecular mass of 17.0 kDa.
Synonyms: IL1B, IL-1, IL1F2, IL1-BETA - Molekulargewicht
- 17.0 kDa
- Gen-ID
- 3553
- NCBI Accession
- NM_000576, NP_000567
- UniProt
- P01584
- Pathways
- NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome
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