VEGF121 Protein (Homodimer)

Produktnummer ABIN1589588

Produktdetails

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Homodimer

Spezies: Human

Quelle: Insektenzellen

Verwendungszweck: VEGF121

Sequenz: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKCDKPR R

Spezifität: Chromosomal location:6p12

Produktmerkmale: Length (aa):121

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 2 - 10 ng/mL.

Kommentare:

Cytokines & Growth Factors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The lyophilized VEGF121 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

Buffer: 50 mM acetic acid

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF121 should be stored in working aliquots at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))

Andere Bezeichnung: VEGF121 (VEGF121 Produkte)

Hintergrund: Human Vascular Endothelial Growth Factor121 (VEGF121), a 18 kDa protein consisting of 121 amino acid residues is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.
Synonyms: vascular endothelial growth factor A 121, VEGFA, VPF, VEGF, MVCD1,

Molekulargewicht: 36 kDa

Gen-ID: 7422

NCBI Accession: NM_001025366, NP_001020537

UniProt: P15692