PDGF-AB Heterodimer (Homodimer) (Active) Protein
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- Target
- PDGF-AB Heterodimer
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- Homodimer
- Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- PDGF-AB
- Sequenz
- Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT
- Spezifität
- Chromosomal location:7p22/22q13.1
- Produktmerkmale
- Length (aa):126/110
- Reinheit
- > 95 % by SDS-PAGE
- Endotoxin-Niveau
- < 0.1 ng per ug of PDGF-AB
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- Applikationshinweise
- The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
- Kommentare
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Cytokines & Growth Factors
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.
- Buffer
- 50 mM acetic acid
- Handhabung
- Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.
- Lagerung
- RT,-20 °C,-80 °C
- Informationen zur Lagerung
- The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AB is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.
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- Target
- PDGF-AB Heterodimer
- Andere Bezeichnung
- PDGF-AB
- Synonyme
- PDGF-A Protein, PDGF1 Protein, platelet derived growth factor subunit A Protein, PDGFA Protein
- Hintergrund
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PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A - Molekulargewicht
- 25.5 kDa
- Gen-ID
- 5154, 5155
- NCBI Accession
- NM_002608, NM_002607, NP_002599, NP_002598
- UniProt
- P04085, P01127
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