PDGF-BB Homodimer (Homodimer) (Active) Protein
(1 reference)-
- Target
- PDGF-BB Homodimer
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- Homodimer
-
Spezies
- Human
-
Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- PDGF-BB
- Sequenz
- SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
- Spezifität
- Chromosomal location:22q13.1
- Produktmerkmale
- Length (aa):109
- Reinheit
- > 95 % by SDS-PAGE
- Endotoxin-Niveau
- < 0.1 ng per ug of PDGF-BB
-
PDGF-BB Homodimer protein
Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 98 % as determined by reducing SDS-PAGE.
PDGF-BB Homodimer (Active) protein (His tag)Spezies: Human Wirt: Hefe Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer (AA 82-190) (Active) proteinSpezies: Human Wirt: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
-
- Applikationshinweise
- The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
- Kommentare
-
Cytokines & Growth Factors
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Centrifuge vial prior to opening. The lyophilized PDGF-BB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.
- Buffer
- 50 mM acetic acid
- Handhabung
- Centrifuge vial prior to opening.
- Lagerung
- RT,-20 °C,-80 °C
- Informationen zur Lagerung
- The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-BB is best stored at -20°C to -70°C.
-
-
: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." in: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).
-
: "Therapeutic Potential of Anti-Angiogenic Multitarget N,O-Sulfated E. Coli K5 Polysaccharide in Diabetic Retinopathy." in: Diabetes, Vol. 64, Issue 7, pp. 2581-92, (2015) (PubMed).
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- Target
- PDGF-BB Homodimer
- Andere Bezeichnung
- PDGF-BB
- Synonyme
- PDGF-2 Protein, PDGF2 Protein, SIS Protein, SSV Protein, c-sis Protein, PDGF-B Protein, Sis Protein, platelet derived growth factor subunit B Protein, platelet derived growth factor, B polypeptide Protein, PDGFB Protein, Pdgfb Protein
- Hintergrund
-
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, INN=Becaplermin - Molekulargewicht
- 24.3 kDa
- Gen-ID
- 5155
- NCBI Accession
- NM_002608, NP_002599
- UniProt
- P01127
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