TIE1 Protein (glycosylated, Homodimer, Soluble) (Fc Tag)

Produktnummer ABIN1589960

Produktdetails

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Protein-Typ: Recombinant

Proteineigenschaft: glycosylated, Homodimer, Soluble

Spezies: Maus

Quelle: CHO Cells

Aufreinigungstag / Konjugat: Dieses TIE1 Protein ist gelabelt mit Fc Tag.

Sequenz: VDLTLLANLR ITDPQRFFLT CVSGEAGAGR SSDPPLLLEK DDRIVRTFPP GQPLYLARNG SHQVTLRGFS KPSDLVGVFS CVGGAGARRT RVLYVHNSPG AHLFPDKVTH TVNKGDTAVL SAHVHKEKQT DVIWKNNGSY FNTLDWQEAD DGRFQLQLQN VQPPSSGIYS ATYLEASPLG SAFFRLIVRG CGAGRWGPGC VKDCPGCLHG GVCHDHDGEC VCPPGFTGTR CEQACREGRF GQSCQEQCPG TAGCRGLTFC LPDPYGCSCG SGWRGSQCQE ACAPDHFGAD CRLQCQCQNG GTCDRFSGCV CPSGWHGVHC EKSDRIPQIL SMATEVEFNI GTMPRINCAA AGNPFPVRGS MKLRKPDGTM LLSTKVIVEP DRTTAEFEVP SLTLGDSGFW ECRVSTSGGQ DSRRFKVNVK VPPVPLTAPR LLAKQSRQLV VSPLVSFSGD GPISSVRLHY RPQDSTIAWS AIVVDPSENV TLMNLKPKTG YNVRVQLSRP GEGGEGGWGP SALMTTDCPE PLLQPWLESW HVEGPDRLRV SWSLPSVPLS GDGFLLRLWD GARGQERREN ISFPQARTAL LTGLTPGTHY QLDVRLYHCT LLGPASPPAH VHLPPSGPPA PRHLHAQALS DSEIQLMWQH PEAPSGPISK YIVEIQVAGG SGDPQWMDVD RPEETSIIVR GLNASTRYLF RVRASVQGLG DWSNTVEEAT LGNGLQSEDP VRESRAAEEG LTRSDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP MLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

Produktmerkmale: Length (AA): 957
Chromosomal location: 4 D2.1, 4 50.0 cM

Reinheit: > 90 % by SDS-PAGE. Visualized by silver stain

Anwendungsinformationen

Kommentare:

Soluble Receptors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The lyophilized sTIE-1/Fc is soluble in water and most aqueous buffers and should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: PBS

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Lyophilized samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted sTIE-1/Fc should be stored in working aliquots at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Andere Bezeichnung: TIE-1 (TIE1 Produkte)

Synonyme: JTK14 Protein, TIE Protein, D430008P04Rik Protein, tie-1 Protein, tyrosine kinase with immunoglobulin like and EGF like domains 1 Protein, tyrosine kinase with immunoglobulin-like and EGF-like domains 1 Protein, tyrosine-protein kinase receptor Tie-1 Protein, endothelium-specific receptor tyrosine kinase 1 Protein, TIE1 Protein, Tie1 Protein, CpipJ_CPIJ018933 Protein, tie1 Protein

Hintergrund: Recombinant murine soluble TIE-1 was fused with the Fc part of human IgG1. The recombinant mature sTIE-1/hFc is a disulfide-linked homodimeric protein. The sTIE-1/hFc monomers have a mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130 kDa protein in SDS-PAGE under reducing conditions. The soluble receptor protein consists of the full extracellular domain (Val23-Glu749). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Murine TIE-1 cDNA encodes a 1134 amino acid (aa) residue precursor protein with an 22 residue putative signal peptide, a 733 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
Synonyms: Tie1, TIE, tie-1, D430008P04Rik

Molekulargewicht: 260.0k Da

NCBI Accession: NP_035717, NM_011587

UniProt: Q06806

Pathways: RTK Signalweg