SDS-PAGE (SDS), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)
Verwendungszweck
E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
Spezifität
E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
Produktmerkmale
Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
Applikationshinweise
Western Blot Dilution: 1:500 - 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
2.0 mg/mL
Buffer
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
Konservierungsmittel
Without preservative
Lagerung
-20 °C
Informationen zur Lagerung
Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.