Optimized DNA sequence encoding extracellular domain of mouse erythropoietin receptor including a C-terminal His tag was expressed in HEK293 cells.
Produktmerkmale
Recombinant mouse EPO receptor is a monomer protein consisting of37 amino acid residue subunits, due to glycosylation migrates as an approximately 35 kDa protein on SDS-PAGE.
Reinheit
> 97 %, as determined by SDS-PAGE and HPLC
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
EPOR
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS solution, pH7.2
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is member of a distinct, structurally-related subclass of CC chemokines. MCP-4/CCL13 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell. Expression of MCP-4/CCL13 mRNA and protein is greater in the sputum, epithelium, submucosal inflammatory cells and bronchoalveolar lavage fluid of asthmatics than in healthy individuals. Involvement of MCPs has also been demonstrated in renal inflammation and atopic dermatitis and the expression of this protein is also correlated with enhanced inflammatory immune responses during immunotherapy