DNA sequence encoding extracellular domain of Mouse IL-10RB fused to a C-terminal polyHis tag was expressed in HEK cells.
Produktmerkmale
Recombinant Mouse Interleukin-10RB is a monomer consisting of12 amino acid residue subunits, and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.
Reinheit
> 90 %, as determined by SDS-PAGE and HPLC
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
IL10RB
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
SDS, WB, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Buffer
PBS solution pH 7.4.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
TNF-beta TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha TNF-beta is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells.