Recombinant Human T-Cell Receptor-Associated Transmembrane Adapter 1/TRAT1 is produced with our E. coli expression system. The target protein is expressed with sequence (Asn29-Asn186) of Human TRAT1 fused with a 6His tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
TRAT1
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, ELISA, SDS
TRAT1
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl,10 % Glycerol, pH 7.4.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
-80 °C
Informationen zur Lagerung
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Haltbarkeit
6 months
Target
TRIM (TRAT1)
(T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))
TCRIM Protein, TRIM Protein, C030046M14Rik Protein, Tcrim Protein, Trim Protein, T cell receptor associated transmembrane adaptor 1 Protein, T-cell receptor associated transmembrane adaptor 1 Protein, TRAT1 Protein, Trat1 Protein
Sub Type
Fusionprotein
Hintergrund
T-Cell Receptor-Associated Transmembrane Adapter 1 (TRAT1) is a single-pass type III membrane protein. TRAT1 exists as a disulfide-linked homodimer and is strongly expressed in the thymus, and to a lesser extent in the spleen, lymph node, and peripheral blood lymphocytes. TRAT1 is phosphorylated on tyrosines by LCK or FYN upon TCR activation. Its function is to stabilizes the TCR (T-cell antigen receptor)/CD3 complex at the surface of T-cells. Alternative Names: T-Cell Receptor-Associated Transmembrane Adapter 1, T-Cell Receptor-Interacting Molecule, TRIM, pp29/30, TRAT1, TCRIM, HSPC062