Recombinant Human Medium-Chain Specific Acyl-CoA Dehydrogenase Mitochondrial/ACADM is produced with our E. coli expression system. The target protein is expressed with sequence (Lys26-Asn421) of Human ACADM fused with a 6His tag at the N-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM Tris, 0.1M NaCl, 20 % Glycerol, pH 8.5.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
-80 °C
Informationen zur Lagerung
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Haltbarkeit
6 months
Target
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
Andere Bezeichnung
ACADM
Synonyme
ACAD1 Protein, MCAD Protein, MCADH Protein, AU018656 Protein, acyl-CoA dehydrogenase medium chain Protein, acyl-Coenzyme A dehydrogenase, medium chain Protein, acyl-CoA dehydrogenase, C-4 to C-12 straight chain Protein, ACADM Protein, Acadm Protein
Sub Type
Fusionprotein
Hintergrund
Medium-Chain Specific Acyl-CoA Dehydrogenase (ACADM) is a mitochondrial fatty acid beta-oxidation that belongs to the acyl-CoA dehydrogenase family. ACADM is a homotetramer enzyme that catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. ACADM is specific for acyl chain lengths of 4 to 16. It is essential for converting these particular fatty acids to energy, especially during fasting periods. Defects in ACADM cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternative Names: Medium-Chain Specific Acyl-CoA Dehydrogenase Mitochondrial, MCAD, ACADM