PARP1 Protein

Produktnummer ABIN1741730

Produktdetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus

Applikation: Western Blotting (WB), ELISA

Produktmerkmale: PARP1 Automodified is highly purified human PARP1, expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow PARP automodification by poly(ADP-ribose). The PARP1 is lyophilized from its native state, and upon reconstitution with water, it retains residual poly ADP-ribosylation enzymatic activity. It is useful as a standard for ELISA, SDS-PAGE, Western blotting, and in other assays.
Full-length hPARP1

Aufreinigung: Affinity chromatography

Reinheit: > 95 %

Anwendungsinformationen

Applikationshinweise: 100 ng/lane WB, 10 ng/well ELISA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconsitute in dH2O

Lagerung: -20 °C

Referenzen

Zhang, Wang, Ding, Yu: "Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome." in: Nature methods, Vol. 10, Issue 10, pp. 981-4, (2013) (PubMed).

Antigendetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: Poly ADP ribose Polymerase 1 (PARP1 Produkte)

Hintergrund: Poly (ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.

Molekulargewicht: >113 kDa

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location