Epigen Protein (AA 24-95)

Produktnummer ABIN2017766

Produktdetails

Target: Epigen (EPGN) (Epithelial Mitogen (EPGN))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 24-95

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 < 1 xg/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1x 10^3 units/mg.
AA 24-95, expressed with an N-terminal Met.

Reinheit: > 95 % by SDS-PAGE analysis.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Epigen (rhEpigen) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhEpigen remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: Epigen (EPGN) (Epithelial Mitogen (EPGN))

Andere Bezeichnung: Epigen (EPGN Produkte)

Synonyme: ALGV3072 Protein, EPG Protein, PRO9904 Protein, 2310069M11Rik Protein, epigen Protein, RGD1560084 Protein, epithelial mitogen Protein, EPGN Protein, Epgn Protein

Hintergrund: Epigen is a cytokine belonging to the Epidermal Growth Factor (EGF) superfamily, which also includes Epiregulin, Amphiregulin, Neuregulin 2-beta, and Transforming Growth Factor alpha. The precursor of Epigen produced in tissues has 154 amino acids, and shares the characteristics of other members of EGF superfamily, including 3 disulfide bonds formed by 6 cysteines. Epigen is present in testis, heart, and liver, and it binds to EGF receptors with a much lower binding affinity than EGF. However, Epigen is more mitogenic than EGF. Epigen achieves its strong mitogenic potency by suppressing ligand-induced receptor inactivation. Unlike EGF, Epigen can also bind to EGF receptors in low pH conditions, helping its recycling. Therefore Epigen has anomalous potency due to its prolonged presence.Recombinant human Epigen (rhEpigen) produced in E. coli is a single non-glycosylated polypeptide chain containing 73 amino acids. A fully biologically active molecule, rhEpigen has a molecular mass of 8.1 kDa analyzed by reducing SDS-PAGE.
Synonyms: EPG, Epithelial mitogen

Molekulargewicht: 8.1 kDa, observed by reducing SDS-PAGE.

Pathways: RTK Signalweg, EGFR Signaling Pathway