ED50 < 20 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0x 10^4 units/mg. AA 40-208, expressed with N-terminal His-Tag.
FGF10
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, SDS, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstituted in ddH2O at 100 μg/mL.
Buffer
Lyophilized after extensive dialysis against PBS.
Lagerung
-80 °C
Informationen zur Lagerung
Lyophilized recombinant human Fibroblast Growth Factor-10 (rhFGF-10) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-10 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares the homolog and receptor FGFR2-IIIb with KGF. However, unlike KGF which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during the multi-organ development via the instructive mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development, and is regulated by Shh during early development. Recombinant human Fibroblast Growth Factor-10 (rhFGF-10) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 21.4 kDa analyzed by reducing SDS-PAGE. Synonyms: Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2