Growth Hormone 1 Protein (GH1) (AA 27-217)

Produktnummer ABIN2017922

Produktdetails

Target: Growth Hormone 1 (GH1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 27-217

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 < 0.5 ng/mL, measured by a cell proliferation assay using Nb2-11 Cells, corresponding to a specific activity of > 2.0x 10^6 units/mg.

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Growth Hormone (rhGH) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhGH should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Referenzen

Basu, Nahar, Kulkarni, Kerekes, Sattler, Hall, Neggers, Holub, Kopchick: "A novel peptide antagonist of the human growth hormone receptor." in: The Journal of biological chemistry, pp. 100588, (2021) (PubMed).

Qian, Basu, Mathes, Arnett, Duran-Ortiz, Funk, Brittain, Kulkarni, Terry, Davis, Singerman, Henry, List, Berryman, Kopchick: "Growth Hormone Upregulates Mediators of Melanoma Drug Efflux and Epithelial-to-Mesenchymal Transition In Vitro and In Vivo." in: Cancers, Vol. 12, Issue 12, (2020) (PubMed).

Basu, Baumgaertel, Wu, Kopchick: "Growth Hormone Receptor Knockdown Sensitizes Human Melanoma Cells to Chemotherapy by Attenuating Expression of ABC Drug Efflux Pumps." in: Hormones & cancer, Vol. 8, Issue 3, pp. 143-156, (2018) (PubMed).

Antigendetails

Target: Growth Hormone 1 (GH1)

Andere Bezeichnung: Growth Hormone (GH) (GH1 Produkte)

Synonyme: GH Protein, GH-N Protein, GHN Protein, IGHD1B Protein, hGH-N Protein, Gh1 Protein, GH1 Protein, gh Protein, Gh Protein, GH2 Protein, RNGHGP Protein, ghl Protein, gh-n Protein, ghn Protein, ighd1b Protein, gh1 Protein, ghb-A Protein, GHI Protein, GHB3 Protein, growth hormone 1 Protein, growth hormone Protein, somatotropin-like Protein, somatotropin Protein, growth hormone prepeptide Protein, growth hormone 1 L homeolog Protein, growth hormone 1 S homeolog Protein, Somatotropin-1 Protein, GH1 Protein, Gh Protein, GH Protein, LOC100305005 Protein, Gh1 Protein, LOC100534452 Protein, LOC100232594 Protein, LOC100303681 Protein, gh1 Protein, LOC100356068 Protein, LOC100136588 Protein, gh1.L Protein, gh1.S Protein, LOC109081196 Protein

Hintergrund: Growth Hormone (GH) is a member of the somatotropin/prolactin family which play an important role in growth control. The human GH cDNA encodes a 217 amino acid (aa), and the first 26 aa is a signal peptide. By alternative splicing, at least four isoforms of GH have been identified. The major role of GH in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. GH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues.Recombinant human Growth Hormone (rhGH) produced in E. coli is a single non-glycosylated polypeptide chain containing 191 amino acids. A fully biologically active molecule, rhGH has a molecular mass of 22.1 kDa analyzed by reducing SDS-PAGE.
Synonyms: GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin

Molekulargewicht: 22.1 kDa, observed by reducing SDS-PAGE.

UniProt: P01241

Pathways: NF-kappaB Signalweg, JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process