Hemagglutinin Protein (HA) (AA 18-529) (His tag)

Produktnummer ABIN2017945

Produktdetails

Target: Hemagglutinin (HA)

Protein-Typ: Recombinant

Proteineigenschaft: AA 18-529

Spezies: Influenza A Virus H1N1

Virus Strain: A/California/04/2009

Quelle: Insect cells (Sf9)

Aufreinigungstag / Konjugat: Dieses Hemagglutinin Protein ist gelabelt mit His tag.

Spezifität: Influenza A H1N1 (A/California/04/2009(H1N1))

Produktmerkmale: AA 18-529, expressed with C-terminus 8*His-Tag.

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin-Niveau: < 1 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower.

Buffer: Lyophilized in 20 mM PB buffer ( pH 7.4), 300 mM NaCl, 5 % mannitol, 5 % trehalose.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant Influenza A H1N1 (A/California/04/2009(H1N1)) Hemagglutinin remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, HA-H1N1 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: Hemagglutinin (HA)

Andere Bezeichnung: Hemagglutinin (HA Produkte)

Synonyme: hemagglutinin Protein, Hemagglutinin Protein, HA Protein, HA Protein, ha Protein

Substanzklasse: Influenza Protein

Hintergrund: Influenza hemagglutinin (HA) is a glycoprotein found on the surface of the influenzavirus. It is responsible for binding the virus to cells with sialic acid on their membranes, such as cells in the upper respiratory tract or erythrocytes. It is also responsible for the fusion of the viral envelope with the endosome membrane after the pH has been reduced. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to clump together in vitro. HA has two functions. First, it allows the recognition of target vertebrate cells, accomplished through binding to these cells' sialic acid-containing receptors. Second, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. H1N1 is a subtype of influenza virus A and the most common cause of influenza in humans. Recombinant Influenza A H1N1 (A/California/04/2009(H1N1)) Hemagglutinin with his-tag produced in Sf9 Cell is a single, glycosylated polypeptide chain containing 520 amino acids. A fully biologically active molecule, HA-H1N1 has a molecular mass of ~66 kDa analyzed by reducing SDS-PAGE.
Synonyms: HA

Molekulargewicht: 66 kDa, observed by reducing SDS-PAGE.