IL-4 Protein (AA 23-140)

Produktnummer ABIN2018019

Produktdetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 23-140

Spezies: Maus

Quelle: CHO Cells

Produktmerkmale: ED50 < 2 ng/mL, measured in a cell proliferation assay using murine HT-2 cells, corresponding to a specific activity of >5 x 10^5 units/mg.

Reinheit: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant Murine Interleukin 4 (IL-4) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: Interleukin 4 (IL-4) (IL4 Produkte)

Hintergrund: Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells[1]. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity[2]. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells[3]. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure[4]. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet[5].
Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1)

Molekulargewicht: 15 kDa, observed by reducing SDS-PAGE.

UniProt: P07750

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation