Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Ra and human IL-20Rb co-transfected murine BaF3 pro-B cells is less than 1.5 ng/mL, corresponding to a specific activity of > 6.7 x 10^5 IU/mg.
Reinheit
> 95 % by SDS-PAGE and HPLC analyses.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
< 1 EU/μg of rHuIL-19 as determined by LAL method.
IL19
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24. Synonyms: Melanoma differentiation association like protein
Molekulargewicht
17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.