Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 5.0-50 ng/mL.
Reinheit
> 96 % by SDS-PAGE and HPLC analyses.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
< 1 EU/μg of rRtMEC/CCL28 as determined by LAL method.
CCL28
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 200 mM NaCl.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
Mucosae-associated Epithelial Chemokine (MEC)/CCL28 (CC chemokine ligand 28) is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Synonyms: MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.
Molekulargewicht
13.1 kDa, a single non-glycosylated polypeptide chain containing 116 amino acids.