ED50 < 80 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL3, corresponding to a specific activity of > 1.25x10^4 units/mg.
CCL3
Spezies: Human
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CCL3
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstituted in ddH2O or PBS at 100 μg/mL.
Buffer
Lyophilized after extensive dialysis against PBS.
Lagerung
-80 °C
Informationen zur Lagerung
Lyophilized recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhMIP-1 alpha remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
MIP-1 alpha/CCL3, also known as LD78 alpha, is an inflammatory chemokine. MIP-1alpha belongs to the CCL chemokine family, and shares 68 % homology with MIP-1beta. The mature form of MIP-1alpha contains 69 amino acids, exists as dimers in solution, and tends to undergo reversible aggregation. The receptors of MIP-1alpha in vivo are mainly the G-protein coupled receptors CCR1 and CCR5. Upon stimulation by endogenous and exogenous agents such as Interleukin-1beta, Interferon-gamma, and lipoteichoic acid from Gram-positive bacteria, monocytes are able to secrete significant amounts of MIP-1alpha. MIP-1alpha augments the adhesions of T lymphocytes, monocytes, and neutrophils to vascular cell adhesion molecule 1. In addition, in wounds, MIP-1alpha chemo-attracts macrophages in order to accelerate the tissue repair process.Recombinant human MIP-1 alpha/CCL3 (rhMIP-1 alpha) produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-1 alpha has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE. Synonyms: Macrophage Inflammatory Protein-1 alpha, CCL3, LD78 alpha