Sonic Hedgehog Protein (SHH) (AA 24-197, Cys24IleIle-Mutant)
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- Target Alle Sonic Hedgehog (SHH) Proteine anzeigen
- Sonic Hedgehog (SHH)
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 24-197, Cys24IleIle-Mutant
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Produktmerkmale
- ED50 < 2.0 μg/mL, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500 units/mg.
- Reinheit
- > 95 % by SDS-PAGE and HPLC analyses.
- Endotoxin-Niveau
- < 0.2 EU/μg, determined by LAL method.
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Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) protein
SHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) protein (His tag)SHH Spezies: Maus Wirt: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Sonic Hedgehog (SHH) (Active) proteinSHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH Spezies: Human Wirt: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Lyophilized recombinant human Sonic Hedgehog (C24II) (rhShh) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhShh should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Haltbarkeit
- 6 months
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- Target
- Sonic Hedgehog (SHH)
- Abstract
- SHH Produkte
- Hintergrund
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Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93 % identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant human Sonic Hedgehog (C24II) (rhShh) produced in E. coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rhShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: Hhg1 - Molekulargewicht
- 19.7 kDa, observed by reducing SDS-PAGE.
- UniProt
- Q15465
- Pathways
- Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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