HMGB1 Protein (AA 2-89)

Produktnummer ABIN2115444

Produktdetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-89

Spezies: Human

Quelle: Escherichia coli (E. coli)

Verwendungszweck: Recombinant Human High Mobility Group Protein B1/HMGB1

Sequenz: GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGETKKKF

Produktmerkmale: Recombinant Human High Mobility Group Protein B1/HMGB1/BOXA is produced by our E. coli expression system. The target protein is expressed with sequence (G2-F89) of Human HMBG1.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mMHepes,500 mMNaCl,0.5 mMDTT, pH 7.9 .

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High Mobility Group Protein B1/HMGB1 (HMGB1 Produkte)

Synonyme: HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein

Hintergrund: HMGB1 belongs to the HMGB family and contains 2 HMG box DNA-binding domains. HMGB1 is a DNA binding proteins that associates with chromatin and has the ability to bend DNA. This nuclear protein organizes the DNA and regulates transcription. After binding HMGB1 bends the DNA, which conducts to the binding of other proteins. HMGB1 suports transcription of many genes in interactions with many transcription factors or cooperation nucleosoms looses packed DNA and increases the chromatin remodeling, contact with core histones changes the structure of nucleosoms. it involved in V(D)J recombination by acting as a cofactor of the RAG complex. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, stays in the nucleus, but hyperacetylation on lysine residues occurs to translocation into the cytosol.
Synonyms: High-mobility group protein B1, high-mobility group protein 1, HMG-1, amphoterin and HMGB1.

Molekulargewicht: 10.5 kDa

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome