FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Proteineigenschaft
AA 36-218
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses FCGR2A Protein ist gelabelt mit His tag.
Marke
MABSol®
Sequenz
AA 36-218
Produktmerkmale
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.2 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
FCGR2A
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
PBS, pH 7.4
Handhabung
Please avoid repeated freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Simonov, Ivanov, Smolov, Abbasova, Piskunov, Poteryaev: "Control of therapeutic IgG antibodies galactosylation during cultivation process and its impact on IgG1/FcγR interaction and ADCC activity." in: Biologicals : journal of the International Association of Biological Standardization, Vol. 58, pp. 16-21, (2019) (PubMed).
Target
FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein
Hintergrund
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.