Adenylate Cyclase (ADCY) (Active) Protein

Produktnummer ABIN2452164

Produktdetails

Target: Adenylate Cyclase (ADCY)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Bordetella pertussis

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Reinheit: > 90 % purity by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: Functionale studies on the cAMP- signaling pathway (10~100 ng/mL)
2) Antigen for western blotting and ELISA
3) SDS-PAGE marker as native adenylate cyclase Activity tested: Addition of the 10~100 ng/mL adenylate cyclase toxin to medium increased significant levels of cellular cAMP levels in CHO and human monocyte cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 550 µg/mL

Buffer: 50 mM Tris-HCl ( pH 8.0), 6 M urea, 150 mM NaCl

Lagerung: -80 °C

Referenzen

Westrop, Hormozi, Da Costa, Parton, Coote: "Bordetella pertussis adenylate cyclase toxin: proCyaA and CyaC proteins synthesised separately in Escherichia coli produce active toxin in vitro." in: Gene, Vol. 180, Issue 1-2, pp. 91-9, (1997) (PubMed).

Friedman: "Bordetella pertussis adenylate cyclase: isolation and purification by calmodulin-sepharose 4B chromatography." in: Infection and immunity, Vol. 55, Issue 1, pp. 129-34, (1987) (PubMed).

Antigendetails

Target: Adenylate Cyclase (ADCY)

Hintergrund: Adenylate cyclase is a toxin produced by a gram-negative coccobacillus, Bordetella pertusis. This toxin penetrates into the animal cells and produces cAMP from ATP in the cytoplasm. It belongs to the RTX family of toxins produced by many gram-negative bacteria. The molecular weight is 177 kDa This product is a recombinant toxin of Bordetella pertussis Tohama strain expressed in E. coli from the structural gene cyaA and its activator gene cyaC and purified by the method of Friedman.

UniProt: P0DKX7