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RuvC Protein

RuvC Spezies: E. coli Wirt: Escherichia coli (E. coli) Native SDS, Func, ELISA, WB Active
Produktnummer ABIN2452186
  • Target Alle RuvC Produkte
    RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
    Protein-Typ
    Native
    Biologische Aktivität
    Active
    Spezies
    E. coli
    Quelle
    • 1
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
    Produktmerkmale
    Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
  • Applikationshinweise
    1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
    2) SDS-PAGE (0. 2 g/lane)
    3) Standard antigen for western blotting and ELISA
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer
    50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
    Lagerung
    -20 °C/-80 °C
    Informationen zur Lagerung
    Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
  • Murayama, Kurokawa, Mayanagi, Iwasaki: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).

    Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).

    Iwasaki, Takahagi, Shiba, Nakata, Shinagawa: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).

  • Target
    RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
    Andere Bezeichnung
    RuvC (RuvC Produkte)
    Synonyme
    ECK1864 Protein, JW1852 Protein, Crossover junction endodeoxyribonuclease RuvC Protein, ruvC Protein
    Hintergrund
    E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
    UniProt
    P0A814
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