HIV1RT Protein

Produktnummer ABIN7233213

Produktdetails

Target: HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human Immunodeficiency Virus (HIV)

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), Dot Blot (DB), Functional Studies (Func), SDS-PAGE (SDS), ELISA

Produktmerkmale: Activity: 5,000 units/mL

Reinheit: > 90 % by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: 1) It is extremely effective for screening new specific inhibitors for HIV virus as a drug for treating AIDS.
2) Generally,Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However,by using this enzyme in combination as an antigen,the detection will be more sensitive.
3) Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
4) Standards for SDS-PAGE

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 50 % glycerol, 40 mM Tris-HCl ( pH 8.3), 50 mM NaCl, 5 mM MgCl2, 0.1 % Triton X-100, 1 mM DTT

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen

Saitoh, Iwasaki, Nakata, Adachi, Shinagawa: "Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme." in: Microbiology and immunology, Vol. 34, Issue 6, pp. 509-21, (1990) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Holtermüller, Bohlen, Castro, Weis: "[Considerations on antacid therapy]." in: Medizinische Klinik, Vol. 72, Issue 30, pp. 1229-41, (1977) (PubMed).

Antigendetails

Target: HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))

Andere Bezeichnung: HIV-1 Reverse Transcriptase (HIV1RT Produkte)

Substanzklasse: Viral Protein

Hintergrund: HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase derived from HIV-1 (AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable for reproduction of AIDS virus. This protein is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified. It is composed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles.