Measurement of the activity: Definition of 1HU is activation of 50 % hemolysis using 3 % sheep red blood cells. The product was highly purified from E.coli over-expressing SLO of Group C hemolytic streptococci. The specific activity is as high as 1,900,000 hemolytic units (HU) /mg and the product forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo.
1) Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent 2) Reagent for membrane pore formation to introduce small-to-macromolecules into living cells
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Inactivated SLO can be reactivated by thiol reagents such as 20 mM cysteine or 10 mM DTT (2)
Konzentration
1 mg/mL
Buffer
PBS, 1 mM DTT, 50 % glycerol
Konservierungsmittel
Dithiothreitol (DTT)
Vorsichtsmaßnahmen
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C/-80 °C
Informationen zur Lagerung
-20 C (For long term storage: -70 C)
Maeda, Ide, Koike, Uchiyama, Kinoshita: "GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." in: Nature cell biology, Vol. 10, Issue 10, pp. 1135-45, (2008) (PubMed).
Walev, Bhakdi, Hofmann, Djonder, Valeva, Aktories, Bhakdi: "Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3185-90, (2001) (PubMed).
Palmer: "The family of thiol-activated, cholesterol-binding cytolysins." in: Toxicon : official journal of the International Society on Toxinology, Vol. 39, Issue 11, pp. 1681-9, (2001) (PubMed).
streptolysin O Protein, Streptolysin O Protein, Thiol-activated cytolysin Protein, slo Protein, M6_Spy0188 Protein
Hintergrund
Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SLO is easily inactivated in the presence of oxygen but can be reactivated by thiol compounds, so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98 %.