Taq SSB (Active) Protein

Produktnummer ABIN2452212

Produktdetails

Target: Taq SSB

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Thermus aquaticus

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Produktmerkmale: The absence of endonucleases and exonucleases was confirmed.

Reinheit: > 95 % of protein determined by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: Stabilizes single-stranded DNA in DNA replication,repair,and recombination

Kommentare:

Activity: Single-stranded DNA binding activity was confirmed.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 50 mM Tris-HCl ( pH 8. 0), 200 mM NaCl, 0. 1 mM dithiothreitol, 0. 5 mM EDTA, 50 % glycerol

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen

Dabrowski, Olszewski, Piatek, Kur: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." in: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).

Antigendetails

Target: Taq SSB

Hintergrund: Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA. It plays important roles in DNA replication and recombination. Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.

UniProt: Q9KH06