Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 μg/mL in the presence of 5 ng/mL of human Myostatin (GDF-8).
GPRASP1
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
GPRASP1
Spezies: Maus
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Handhabung
As with any protein, exposing GASP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Lagerung
-20 °C
Informationen zur Lagerung
The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted GASP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
Haltbarkeit
24 months
Target
GPRASP1
(G Protein-Coupled Receptor Associated Sorting Protein 1 (GPRASP1))
pips Protein, 2210415K24Rik Protein, 3110031O14Rik Protein, C87852 Protein, GASP Protein, GASP1 Protein, GASP-1 Protein, G protein-coupled receptor associated sorting protein 1 Protein, Gprasp1 Protein, GPRASP1 Protein
Hintergrund
Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-beta binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66 kDa by SDS-PAGE analysis under non-reducing conditions.