TFPI Protein (His tag)
-
- Target Alle TFPI Proteine anzeigen
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
-
Spezies
- Human
-
Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses TFPI Protein ist gelabelt mit His tag.
- Applikation
- Western Blotting (WB)
- Produktmerkmale
-
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2. The IC50 value is < 0. 35 nM, as measured in 100 μL reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0. 05 % Brij-35 (w/v), pH 7. 5.
- Reinheit
- >90 % as determined by SDS-PAGE.
- Top Product
- Discover our top product TFPI Protein
-
-
- Applikationshinweise
- This recombinant protein can be used for WB. For research use only.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Lagerung
- -80 °C,-20 °C
- Informationen zur Lagerung
- Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
-
- Target
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- Andere Bezeichnung
- TFPI (TFPI Produkte)
- Synonyme
- EPI Protein, LACI Protein, TFI Protein, TFPI1 Protein, A630013F22Rik Protein, AW552122 Protein, TFPI Protein, si:dz44f8.1 Protein, tfpib Protein, wu:fb92g01 Protein, zgc:152844 Protein, zgc:158592 Protein, tissue factor pathway inhibitor Protein, tissue factor pathway inhibitor a Protein, TFPI Protein, Tfpi Protein, tfpia Protein
- Hintergrund
- Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF), The second K domain inhibits factor Xa, The third K domain binds to heparin, The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
- Molekulargewicht
- 30 kDa
- Gen-ID
- 7035
- NCBI Accession
- NP_006278
- UniProt
- P10646
-