FGF6 Protein (AA 41-208, N-Term)

Produktnummer ABIN2666435

Dieses Recombinant FGF6-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für FGF6 Protein (AA 41-208, N-Term) (ABIN2666435)

Target: FGF6 (Fibroblast Growth Factor 6 (FGF6))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: N-Term, AA 41-208

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: The ED50 is 0.02 - 0.1 ng/mL, corresponding to a specific activity 1.0 - 5.0 x 107 units/mg, as determined by a dose-dependent proliferation of NIH3T3 cells in the presence of 1.5 μg/mL heparin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2, 10 % Glycerol and 1 % CHAPS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Human FGF-6 is not stable at 37°C. Unopened vials can be stored between 2°C and 8°C for one week, at -20°C for three months, or at -70°C for 6 months.

Antigendetails

Target: FGF6 (Fibroblast Growth Factor 6 (FGF6))

Andere Bezeichnung: FGF-6

Hintergrund: FGF-6 is a member of the fibroblast growth factor (FGF) family, and it is closely related in structure to FGF-4. FGF-6 exhibits a very restricted expression profile predominantly in the developing muscle in embryos. FGF-6 mRNA can also be detected in testis, heart, and skeletal muscle in adults. FGF-6 is involved in both proliferation and differentiation of the myogenic lineages. In vitro studies have shown that the effects of FGF-6 are concentration dependent. At lower concentration, FGF-6 can increase the expression of many muscle cell differentiation markers. At higher concentrations, FGF-6 can downregulate the expression of myogenic markers and some myogenic transcription factors, and can delay C2 myogenic cell differentiation. Injection of FGF-6 recombinant protein into damaged mouse muscle can induce cyclin D1 mRNA and differentiation markers, including Cdkl1, MHC I and Tn1, which results in the acceleration of muscle regeneration. It has been shown that the expression of FGF-6 can transiently be upregulated by muscle injuries in both fast and slow myofibers. FGF-6 can stimulate migration of myogenic stem cells and also control the maintenance of muscle progenitor cells. During muscle regeneration, it has been shown that FGF-6 can be released from necrotic myofibers and be sequestered by basal laminae. FGF-6 regulates muscle differentiation through a calcineurin-dependent pathway and regulates myofiber size through IGF-II/TGF2R pathway. FGF-6 is also involved in bone metabolism.

Molekulargewicht: The 169 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kDa. The DTT-reduced and non-reduced protein migrates at approximately 20 kDa by SDS-PAGE. The predicted N-terminal amino acid is Met.

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung