IL23 Protein (AA 21-189, AA 23-328)
-
- Target Alle IL23 Proteine anzeigen
- IL23 (Interleukin 23 (IL23))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 21-189, AA 23-328
-
Spezies
- Human
-
Quelle
- Insektenzellen
- Applikation
- Intracellular Flow Cytometry (ICFC)
- Reinheit
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
-
Less than 0.01 ng per μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product IL23 Protein
-
-
- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
-
Biological activity: ED50=0.4 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.5 x 106 units/mg as determined by mouse splenocyte IL-17A secretion, which is induced by hIL-23 in a dose dependent manner.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Rekonstitution
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in buffer containing carrier protein such as 1 % BSA or HSA or 10 % FBS. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- Buffer
- 0.22 μm filtered protein solution is in PBS.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unopened vial can be stored at -20°C for three months or at -70°C for six months.
-
- Target
- IL23 (Interleukin 23 (IL23))
- Andere Bezeichnung
- IL-23 (IL23 Produkte)
- Synonyme
- IL-23 Protein, p19 Protein, SGRF Protein, il23p19 Protein, interleukin 23, alpha subunit p19 Protein, interleukin 23 subunit alpha Protein, Il23a Protein, IL23A Protein
- Hintergrund
- Interleukin 23 (IL-23) is a member of the IL-6 family of cytokines, and it is comprised of two subunits, p19 and p40. The p19/p40 heterodimer is stabilized by a disulfide bond. The p40 subunit is shared by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells, p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rb1 and IL-23R. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling molecules. JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5.
- Molekulargewicht
- The total predicted molecular weight is 53.3 Da. The non-reduced protein migrates at approximately 60 kDa and the DTT-reduced protein produces two bands at approximately 19 kDa and 40 kDa by SDS-PAGE.
-