GZMB Protein (AA 17-247)

Produktnummer ABIN2666523

Produktdetails

Target: GZMB (Granzyme B (GZMB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 17-247

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 1.0 EU per μg of protein as determine by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Recombinant mouse Granzyme B supplied in its inactive form. Once the mouse Granzyme B is activated by active mouse Capthepsin C/DPPI, it is able to cleave the peptide substrate t-Butyloxycaronyl-Ala-Ala-ThioBenzyl ester (Boc-AAD-SBzl) in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB)1,2, with an activity > 3000 pmol/min/μg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The recombinant protein could be aliquoted and stored at -20 °C in a sterile buffer (20 mM Tris, 150 mM NaCl, and pH 7.5).

Konzentration: 200 μg/mL

Buffer: 0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, and pH 7.5.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -70°C for six months.

Antigendetails

Target: GZMB (Granzyme B (GZMB))

Andere Bezeichnung: Granzyme B (GZMB Produkte)

Synonyme: CCPI Protein, CGL-1 Protein, CGL1 Protein, CSP-B Protein, CSPB Protein, CTLA1 Protein, CTSGL1 Protein, HLP Protein, SECT Protein, granzyme B Protein, GZMB Protein

Hintergrund: Granzyme B is a serine protease expressed by cytotoxic T cells (CTL) and NK cells. Its main function is to induce cell death to eliminate harmful targets such as allogeneic, virally infected, and tumorous cells. This is evident by the fact that CTLs from mice deficient of granzyme B exhibit a profound defect in inducing rapid DNA fragmentation and apoptosis in target cells. Following receptor-mediated conjugate formation between CTL or NK and their target cell, granzyme B enters the target via endocytosis, and subsequently activates multiple protein substrates to induce apoptosis. Most circulating CD56+ CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzyme A and B. In contrast, few circulating CD4+ T cells express granzymes A or B. Activation of CD8+ and CD4+ T cells induces substantial expression of granzyme B, but not granzyme A. Besides CTL and NK, evidence has shown that the distribution of human granzyme B has a broader spectrum of cells including CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, and B cells. Although its role in cytotoxic lymphocyte-mediated apoptosis is well established, granzyme B can also degrade extracellular matrix proteins and alter inflammation if present in the extracellular milieu. These findings suggest that granzyme B can function as an activation molecule with potentially important immunoregulatory functions. In addition, it was shown that expression of granzyme B is elevated in acute coronary syndrome and acute myocardia infarction, indicating that granzyme B could be a factor involved in cardiovascular diseases.

Molekulargewicht: This 246 amino acid recombinant protein has a predicted molecular mass of approximately 27.5 kDa. The protein migrates at approximately 37 kDa in DTT-reducing conditions and approximately 37 kDa in non-reducing conditions by SDS-PAGE. The predicted N-term

Pathways: Apoptose, Caspase Kaskade in der Apoptose