CCL17 Protein (AA 24-94)

Produktnummer ABIN2666600

Produktdetails

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 24-94

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR4 transfectans cells in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Andere Bezeichnung: CCL17 (CCL17 Produkte)

Hintergrund: CCL17 was initially isolated from phytohemagglutinin-stimulated PBMC. CCL17 is constitutively expressed in the thymus and, under activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA+ CD4+ T cells plays a key role in allergic diseases like atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. Also, CCL17 and CCL22 secreted by DC seem to mediate the recruitment of regulatory T cells to sites of inflammation in patients with chronic hepatitis. CCL17 is upregulated by several combinations of cytokines: TNFα, IL-4, and IFNγ in bronchial epithelial cells, TGFβ1, TNFα, and IFNγ in human keratinocyte cell lines, and histamine and prostaglandin E2P in immature DC.

Molekulargewicht: The 71 amino acid recombinant protein has a predicted molecular mass of approximately 8 kDa. The DTT-reduced protein migrates at approximately 10 kDa and non-reduced protein migrates at approximately 11 kDa by SDS-PAGE. The N-terminal amino acid is Ala.