LIF Protein (AA 23-202)
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- Target Alle LIF Proteine anzeigen
- LIF (Leukemia Inhibitory Factor (LIF))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 23-202
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Immunofluorescence (IF)
- Reinheit
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- Endotoxin-Niveau
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product LIF Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Biological activity: ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized, carrier-free.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- LIF (Leukemia Inhibitory Factor (LIF))
- Andere Bezeichnung
- LIF (LIF Produkte)
- Synonyme
- CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein
- Hintergrund
- Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF displays diverse biological effects but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. Human and mouse LIF share 79 % sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulation of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF can be upregulated by pro-inflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.
- Molekulargewicht
- The 180 amino acid recombinant protein has a predicted molecular mass of 19.6 kDa. The predicted N-terminal amino acid is Ser.
- Pathways
- JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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