Neurotrophin 4 Protein (NTF4) (AA 81-210)

Produktnummer ABIN2667564

Produktdetails

Target: Neurotrophin 4 (NTF4)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 81-210

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >98 % , as determined by SDS-PAGE and HPLC analysis.

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: The ED50, determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forbrain primary septal cell cultures, was found to be in the range of 20-50 ng/ml, corresponding to a specific activity of 2-5 x 104 units/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: Neurotrophin 4 (NTF4)

Andere Bezeichnung: NT-4 (NTF4 Produkte)

Hintergrund: Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. Structure related proteins include neuron growth factor (NGF), brain derived neurogenic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4). They are highly homologous and share a similar structure with a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous systems and play important roles in injury induced neuron regeneration. NT-4 is encoded by the NTF4 gene. The name NT-4/5 arose from the uncertainties between neurotrophin-5 found in humans and neurotrophin-4 identified in Xenopus. Although NT-4 is structurally the most divergent neurotrophin, like BNDF NT-4 binds to receptor TrkB with high affinity and mediates similar functions with BDNF in neurogenesis. However, similar to NGF and NT-3 gene-knockouts, the disruption of BDNF function severely affects neuron development. However, NT-4 knockout mice show minor deficits, with nearly 50 % neuron loss in nodose-petrosal and geniculate ganglia. The double knockouts of BDNF and NT-4 present additive deficit phenotype compared to NT-4 knockout mice. It is thus suggested that there are two groups of downstream target cells through multiple coreceptors interaction of BDNF/TrkB and NT-4/TrkB signaling. There are also some exceptions of biological actions of NT-4 that are not parallel with BDNF function. NT-4, but not BDNF, is reported to promote survival of striatal neurons and is able to overturn spatial memory impairments in aged rats. NT-4 is also suggested to participate in stem cell differentiation toward oligodendrocyte progenitor cells in the brain.

Molekulargewicht: NT-4 recombinant protein is a noncovalent linked homodimer. The 130 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa as monomer, 28 kDa as dimer. The predicted N-terminal Amino acid is Gly.

Pathways: RTK Signalweg