Annexin V Protein (FITC)

Produktnummer ABIN2669257

Dieses Annexin V-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Annexin V Protein (FITC) (ABIN2669257)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Spezies: Säugetier

Quelle: Escherichia coli (E. coli)

Applikation: Biochemical Assay (BCA)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Annexin V Protein ist gelabelt mit FITC.

Produktmerkmale: Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (cat. no. 420403/420404) or Propidium Iodide Solution (cat. no. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Aufreinigung: The purified protein was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Bestandteile: FITC Annexin V

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from prolonged exposure to light. Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: The Annexin V solution should be stored undiluted between 2°C and 8°C.

Antigendetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Andere Bezeichnung: Annexin V

Pathways: Apoptose