Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.
Aufreinigung
Avidin is conjugated with horseradish peroxidase under optimal conditions.