NR1I2
(Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))
Protein-Typ
Recombinant
Proteineigenschaft
full length
Spezies
Human
Quelle
Baculovirus
Applikation
Western Blotting (WB), Electrophoretic Mobility-Shift Assay (EMSA), Protein Interaction (PI)
Produktmerkmale
Recombinant PXR (accession number NM_003889) is expressed in a baculovirus system, carries the full-length coding sequence and is purified by an affinity column in combination with FPLC chromatography.
Aufreinigung
Purified by an affinity column in combination with FPLC chromatography.
NR1I2
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
NR1I2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Applikationshinweise
PXR can be used for Western blot (50 kDa), gel mobility shift assay, for protein-protein interaction assay and small molecule-protein interaction assays. 1 ng is sufficient for a gel mobility shift assay in a 20 μL reaction, 100 ng is sufficient for a protein-protein interaction assay detected by immuno-blot system. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.3 μg/μL
Target
NR1I2
(Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))