FAK (PTK2)
(PTK2 Protein tyrosine Kinase 2 (PTK2))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Proteineigenschaft
AA 2-1052, GST-His-Tag
Spezies
Human
Quelle
Baculovirus
Aufreinigungstag / Konjugat
Dieses FAK Protein ist gelabelt mit GST-His-Tag.
Applikation
Western Blotting (WB), Kinase Activity Assay (KAA)
Produktmerkmale
Recombinant human FAK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids A2-H1052, accession number L13616 and N-terminally fused to GST-HIS6TagTagTag-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
PTK2
Spezies: Human, Ratte, Maus
Wirt: Escherichia coli (E. coli)
Recombinant
WB, PC
Applikationshinweise
Recombinant FAK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~145.394 kDa. The activity of the protein is ~ 1.5 pmol/μg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 μg/50 μL PEG20.000, Substrate: PolyEY 0.5 μg/50 μL, Recombinant FAK: 200 ng/50 μL. Kinase activity may vary depending on the substrate and reaction conditions used.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.135 μg/μL
Target
FAK (PTK2)
(PTK2 Protein tyrosine Kinase 2 (PTK2))