Recombinant PRMT6 (accession number NP_060607.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 43.7 kDa.
PRMT6
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS
Recombinant PRMT6 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl Histone H3 purified from E. coli (rH3), 30 ng/μl Recombinant PRMT6 protein at 2 hours at room temperature. Activity was detected by fluorography.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Lagerung
-80 °C
Informationen zur Lagerung
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
CG9927 Protein, DART6 Protein, Dmel\\CG9927 Protein, PRMT6 Protein, HRMT1L6 Protein, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 6 Protein, ATPRMT6 Protein, protein arginine methyltransferase 6 Protein, DKFZp459B1431 Protein, Hrmt1l6 Protein, hrmt1l6 Protein, hrmt6 Protein, im:6908706 Protein, AW124876 Protein, BB233495 Protein, Arginine methyltransferase 6 Protein, protein arginine methyltransferase 6 Protein, protein arginine methyltransferase 6 S homeolog Protein, arginine N-methyltransferase, type I Protein, protein arginine N-methyltransferase 6 Protein, Art6 Protein, PRMT6 Protein, prmt6.S Protein, prmt6 Protein, Prmt6 Protein
Hintergrund
PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors.