Recombinant Smyd3 (accession number NP_001161212.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 50.7 kDa.
SMYD3
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, ELISA, SDS
Recombinant Smyd3 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Specifically mediates the methylation of 'Lys-4' and 'Lys-5' of histone H3 and may methylate non-histone substrates. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl non-histone peptide, 30 ng/μl Recombinant Smyd3 protein at 2 hours at room temperature. Activity was detected by fluorography.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Lagerung
-80 °C
Informationen zur Lagerung
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
KMT3E Protein, ZMYND1 Protein, ZNFN3A1 Protein, bA74P14.1 Protein, 2410008A19Rik Protein, Zmynd1 Protein, SET and MYND domain containing 3 Protein, SMYD3 Protein, Smyd3 Protein
Hintergrund
SET and MYND domain-containing protein 3 (Smyd3) is a member of the Smyd family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and tri-methylation, but not monomethylation. Smyd3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Smyd3 expression has been shown to be upregulated in certain cancers.