Dieses BRD1 Protein ist gelabelt mit His tag,DYKDDDDK Tag.
Applikation
Binding Studies (Bind), Screening Assay (ScA)
Produktmerkmale
The peptide corresponding to amino acids 556-688 that contains the bromodomain sequences of BRD1 (accession number NP_055392.1) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 21.5 kDa.
BRD1
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
Applikationshinweise
Recombinant BRD1 (556-688) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Lagerung
-80 °C
Informationen zur Lagerung
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
Bromodomain-containing protein 1 (BRD1) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of several members that function in the regulation of transcriptional activation and chromatin remodeling.