DNMT3A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2719617

Produktdetails

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 3

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses DNMT3A Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human DNMT3A (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:,DNMT3A activity verified in a biochemical assay:

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Cree, Fredericks, Miller, Pearce, Filichev, Fee, Kennedy: "DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." in: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).

Antigendetails

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Andere Bezeichnung: Dnmt3a (DNMT3A Produkte)

Synonyme: DNMT3A Protein, DNMT3A2 Protein, M.HsaIIIA Protein, MmuIIIA Protein, dnmt3a1 Protein, dnmt6 Protein, DNA methyltransferase 3 alpha Protein, DNA methyltransferase 3A Protein, DNA (cytosine-5-)-methyltransferase 3 alpha b Protein, DNMT3A Protein, Dnmt3a Protein, dnmt3a Protein, dnmt3ab Protein

Hintergrund: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Molekulargewicht: 101.7 kDa

NCBI Accession: NP_072046