IL12A Protein (Transcript Variant 2)

Produktnummer ABIN2723598

Produktdetails

Target: IL12A (Interleukin 12 alpha (IL12A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 2

Spezies: Maus

Quelle: CHO Cells

Applikation: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-12 alpha / IL12A (transcript variant 2) protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Assay #1: ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is > 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg. Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL12A (Interleukin 12 alpha (IL12A))

Andere Bezeichnung: Interleukin-12 Alpha,il12a (IL12A Produkte)

Synonyme: CLMF Protein, IL-12A Protein, NFSK Protein, NKSF1 Protein, P35 Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p35 Protein, Il-12 p35 Protein, IL12p40 Protein, IL12 Protein, IL-12 p35 Protein, interleukin 12A Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12A Protein, IL12B Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Molekulargewicht: 75 kDa

NCBI Accession: NP_032377

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation