Isocitrate Dehydrogenase Protein (IDH) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2723840

Produktdetails

Target: Isocitrate Dehydrogenase (IDH)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Isocitrate Dehydrogenase Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Isocitrate dehydrogenase / IDH protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Isocitrate Dehydrogenase (IDH)

Andere Bezeichnung: Isocitrate Dehydrogenase,idh (IDH Produkte)

Synonyme: CG7176 Protein, CT22171 Protein, Dmel\\CG7176 Protein, IDH Protein, IDH-NADP Protein, Idh-NADP Protein, idh Protein, isocitrate dehydrogenase Protein, l(3)L3852 Protein, F20D21.16 Protein, F20D21_16 Protein, SMU.672 Protein, icd Protein, Isocitrate dehydrogenase Protein, isocitrate dehydrogenase Protein, isocitrate dehydrogenase, NADP-dependent Protein, Monomeric isocitrate dehydrogenase Protein, ankyrin repeat domain 26-like Protein, Idh Protein, ICDH Protein, idh Protein, icd Protein, SSO_RS10660 Protein, JK_RS08640 Protein, LOC618625 Protein

Hintergrund: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molekulargewicht: 46 kDa

NCBI Accession: NP_005887